BLAST help

A brief introduction to BLAST
BLAST (Basic Local Alignment Search Tool) is the heuristic search algorithm employed by the programs blastp, blastn, blastx, tblastn, and tblastx; these programs ascribe significance to their findings using the statistical methods of Karlin and Altschul (1990, 1993) with a few enhancements. The BLAST programs were tailored for sequence similarity searching -- for example to identify homologs to a query sequence.
For further details, see the NCBI BLAST tutorial.

The programs are :

Availability in NPS@
These programs are available :

So, for example, you can use BLAST to find similar sequences in a database built from an ACNUC query or after a PATTINPROT search.

Parameters
BLAST parameters are not currently available for the user.
By default the number of description and the number of alignment are set to 500.
The expected threshold is set to 10.0.
The comparison matrix is BLOSUM62.
The default behavior is selected for gap opening (11) and gap extending (1) costs.
To retrieve these informations see the line "Information and statistics : [BLASTP]" in NPS@ BLAST result file.

NPS@ BLAST output example
The NPS@ BLAST output is divided into three parts.

References


User : public@52.91.245.237. Last modification time : Thu Jan 14 19:00:29 2016. Current time : Thu Dec 13 20:16:59 2018